rnascope probes against rbfox3 (neun Search Results


gene exp rbfox3 mm01248771 m1  (Thermo Fisher)
99
Thermo Fisher gene exp rbfox3 mm01248771 m1
List of reagents used for immunohistochemistry, in situ hybridization, and qPCR.
Gene Exp Rbfox3 Mm01248771 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rnascope+probes+against+rbfox3+(neun/Gene+Exp%2E+Rbfox3%2C+Mm01248771_m1/pmc05787097-29-2--1
Average 99 stars, based on 1 article reviews
gene exp rbfox3 mm01248771 m1 - by Bioz Stars, 2026-09
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anti neuronal nuclei neun  (Proteintech)
96
Proteintech anti neuronal nuclei neun
Whole‐brain expression analysis of VDBP mRNA or protein in wild‐type, CUMS‐susceptible, and CUMS‐resilient mice. a) C57BL/6J mouse brains (male mice 6–8 weeks old) were serially sectioned into 30 µm slices and immunostained with VDBP antibody. b) Representative immunofluorescence images of VDBP protein expression in various brain regions. Green: VDBP. Blue: DAPI. Scale bar = 20 or 100 µm. c) Fluorescence intensity of VDBP in each brain area of wild‐type mice. n = 4 mice per group. d) Experimental timeline for CUMS paradigm, behavioral tests, and preparation of brain sections. e–h) Behavioral test results for control and CUMS mice. CUMS susceptible mice showed lower sucrose preference (e), a decreased central zone duration time in the OFT (f), and increased immobility time in the TST (g) and FST (h). CUMS resilient mice behaved indistinguishably from control nonstressed mice ( n = 10 mice per group). i) Fluorescence intensity of VDBP in each brain area of susceptible and resilient mice after the CUMS paradigm ( n = 4 mice per group). j) Representative immunofluorescence images of VDBP protein expression in emotion‐related brain regions in control, CUMS‐susceptible, and CUMS‐resilient mice. Scale bar = 100 µm (left) and 20 µm (right). k) Fluorescence intensity of VDBP in emotion‐related brain regions in control, CUMS‐susceptible, and CUMS‐resilient mice ( n = 4 mice per group). l,m) Representative confocal images and quantification of VDBP RNAscope signal colocalized with cell‐specific markers for neurons <t>(NeuN),</t> astrocytes (GFAP), and microglia (Iba1) in the PrL of control, CUMS‐susceptible, and CUMS‐resilient mice. n = 4 mice. Scale bar = µm 20 (left) and 10 µm (right). n,o) Representative confocal images (n) and quantification (o) of VDBP protein colocalized with cell‐specific markers for neurons (NeuN), astrocytes (GFAP), and microglia (Iba1) in the PrL of control, CUMS‐susceptible, and CUMS‐resilient mice. n = 4 mice. Scale bar = 20 µm (left) and 10 µm (right). Data represent the mean ± SEM. For comparisons among groups, one‐way analysis of variance (ANOVA) followed by Bonferroni post hoc tests was used. * p < 0.05, ** p < 0.01, *** p < 0.001.
Anti Neuronal Nuclei Neun, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rnascope+probes+against+rbfox3+(neun/NeuN+Antibody/pmc11809382-251-48-52
Average 96 stars, based on 1 article reviews
anti neuronal nuclei neun - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier
nbp3  (Novus Biologicals)
93
Novus Biologicals nbp3
Whole‐brain expression analysis of VDBP mRNA or protein in wild‐type, CUMS‐susceptible, and CUMS‐resilient mice. a) C57BL/6J mouse brains (male mice 6–8 weeks old) were serially sectioned into 30 µm slices and immunostained with VDBP antibody. b) Representative immunofluorescence images of VDBP protein expression in various brain regions. Green: VDBP. Blue: DAPI. Scale bar = 20 or 100 µm. c) Fluorescence intensity of VDBP in each brain area of wild‐type mice. n = 4 mice per group. d) Experimental timeline for CUMS paradigm, behavioral tests, and preparation of brain sections. e–h) Behavioral test results for control and CUMS mice. CUMS susceptible mice showed lower sucrose preference (e), a decreased central zone duration time in the OFT (f), and increased immobility time in the TST (g) and FST (h). CUMS resilient mice behaved indistinguishably from control nonstressed mice ( n = 10 mice per group). i) Fluorescence intensity of VDBP in each brain area of susceptible and resilient mice after the CUMS paradigm ( n = 4 mice per group). j) Representative immunofluorescence images of VDBP protein expression in emotion‐related brain regions in control, CUMS‐susceptible, and CUMS‐resilient mice. Scale bar = 100 µm (left) and 20 µm (right). k) Fluorescence intensity of VDBP in emotion‐related brain regions in control, CUMS‐susceptible, and CUMS‐resilient mice ( n = 4 mice per group). l,m) Representative confocal images and quantification of VDBP RNAscope signal colocalized with cell‐specific markers for neurons <t>(NeuN),</t> astrocytes (GFAP), and microglia (Iba1) in the PrL of control, CUMS‐susceptible, and CUMS‐resilient mice. n = 4 mice. Scale bar = µm 20 (left) and 10 µm (right). n,o) Representative confocal images (n) and quantification (o) of VDBP protein colocalized with cell‐specific markers for neurons (NeuN), astrocytes (GFAP), and microglia (Iba1) in the PrL of control, CUMS‐susceptible, and CUMS‐resilient mice. n = 4 mice. Scale bar = 20 µm (left) and 10 µm (right). Data represent the mean ± SEM. For comparisons among groups, one‐way analysis of variance (ANOVA) followed by Bonferroni post hoc tests was used. * p < 0.05, ** p < 0.01, *** p < 0.001.
Nbp3, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rnascope+probes+against+rbfox3+(neun/RBFOX3%2FNeuN+Antibody/pm40154477-234-36-41
Average 93 stars, based on 1 article reviews
nbp3 - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier

Image Search Results


List of reagents used for immunohistochemistry, in situ hybridization, and qPCR.

Journal: Frontiers in Neuroanatomy

Article Title: Leptin Receptor Expression in Mouse Intracranial Perivascular Cells

doi: 10.3389/fnana.2018.00004

Figure Lengend Snippet: List of reagents used for immunohistochemistry, in situ hybridization, and qPCR.

Article Snippet: Rbfox3 , Mm01248771_m1 , , , .

Techniques: Immunohistochemistry, In Situ Hybridization, RNAscope

qPCR analysis of the mediobasal hypothalamus (H) and dura mater meninges (M) of LepRb-deficient mice (red) and control littermates (white). (A–C) Expression of different isoforms of the leptin receptors including Lepr mRNA (all isoform), Lepr(a) (short-isoform), and Lepr(b) (long isoform) in the hypothalamus and meninges. (D–G) Expression of select markers of mural cells in the hypothalamus and meninges including vitronectin (Vtn), Platelet-derived growth factor receptor beta (Pdgfrb), Alpha-actin-1 (Acta1), and Chondroitin Sulfate Proteoglycan (Cspg4). (H–J) Expression of different neuronal genes in the hypothalamus and meninges including RNA Binding Protein Fox-1 Homolog 3 (Rbfox3), Pomc (Pro-opiomelanocortin), and Neuropeptide Y (Npy). The value above each bar graph is the cycle number at threshold. Asterisks identify results that were significantly different from chow at p < 0.05, p < 0.01, and p < 0.001.

Journal: Frontiers in Neuroanatomy

Article Title: Leptin Receptor Expression in Mouse Intracranial Perivascular Cells

doi: 10.3389/fnana.2018.00004

Figure Lengend Snippet: qPCR analysis of the mediobasal hypothalamus (H) and dura mater meninges (M) of LepRb-deficient mice (red) and control littermates (white). (A–C) Expression of different isoforms of the leptin receptors including Lepr mRNA (all isoform), Lepr(a) (short-isoform), and Lepr(b) (long isoform) in the hypothalamus and meninges. (D–G) Expression of select markers of mural cells in the hypothalamus and meninges including vitronectin (Vtn), Platelet-derived growth factor receptor beta (Pdgfrb), Alpha-actin-1 (Acta1), and Chondroitin Sulfate Proteoglycan (Cspg4). (H–J) Expression of different neuronal genes in the hypothalamus and meninges including RNA Binding Protein Fox-1 Homolog 3 (Rbfox3), Pomc (Pro-opiomelanocortin), and Neuropeptide Y (Npy). The value above each bar graph is the cycle number at threshold. Asterisks identify results that were significantly different from chow at p < 0.05, p < 0.01, and p < 0.001.

Article Snippet: Rbfox3 , Mm01248771_m1 , , , .

Techniques: Control, Expressing, Derivative Assay, RNA Binding Assay

Whole‐brain expression analysis of VDBP mRNA or protein in wild‐type, CUMS‐susceptible, and CUMS‐resilient mice. a) C57BL/6J mouse brains (male mice 6–8 weeks old) were serially sectioned into 30 µm slices and immunostained with VDBP antibody. b) Representative immunofluorescence images of VDBP protein expression in various brain regions. Green: VDBP. Blue: DAPI. Scale bar = 20 or 100 µm. c) Fluorescence intensity of VDBP in each brain area of wild‐type mice. n = 4 mice per group. d) Experimental timeline for CUMS paradigm, behavioral tests, and preparation of brain sections. e–h) Behavioral test results for control and CUMS mice. CUMS susceptible mice showed lower sucrose preference (e), a decreased central zone duration time in the OFT (f), and increased immobility time in the TST (g) and FST (h). CUMS resilient mice behaved indistinguishably from control nonstressed mice ( n = 10 mice per group). i) Fluorescence intensity of VDBP in each brain area of susceptible and resilient mice after the CUMS paradigm ( n = 4 mice per group). j) Representative immunofluorescence images of VDBP protein expression in emotion‐related brain regions in control, CUMS‐susceptible, and CUMS‐resilient mice. Scale bar = 100 µm (left) and 20 µm (right). k) Fluorescence intensity of VDBP in emotion‐related brain regions in control, CUMS‐susceptible, and CUMS‐resilient mice ( n = 4 mice per group). l,m) Representative confocal images and quantification of VDBP RNAscope signal colocalized with cell‐specific markers for neurons (NeuN), astrocytes (GFAP), and microglia (Iba1) in the PrL of control, CUMS‐susceptible, and CUMS‐resilient mice. n = 4 mice. Scale bar = µm 20 (left) and 10 µm (right). n,o) Representative confocal images (n) and quantification (o) of VDBP protein colocalized with cell‐specific markers for neurons (NeuN), astrocytes (GFAP), and microglia (Iba1) in the PrL of control, CUMS‐susceptible, and CUMS‐resilient mice. n = 4 mice. Scale bar = 20 µm (left) and 10 µm (right). Data represent the mean ± SEM. For comparisons among groups, one‐way analysis of variance (ANOVA) followed by Bonferroni post hoc tests was used. * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: Advanced Science

Article Title: Microglia‐Derived Vitamin D Binding Protein Mediates Synaptic Damage and Induces Depression by Binding to the Neuronal Receptor Megalin

doi: 10.1002/advs.202410273

Figure Lengend Snippet: Whole‐brain expression analysis of VDBP mRNA or protein in wild‐type, CUMS‐susceptible, and CUMS‐resilient mice. a) C57BL/6J mouse brains (male mice 6–8 weeks old) were serially sectioned into 30 µm slices and immunostained with VDBP antibody. b) Representative immunofluorescence images of VDBP protein expression in various brain regions. Green: VDBP. Blue: DAPI. Scale bar = 20 or 100 µm. c) Fluorescence intensity of VDBP in each brain area of wild‐type mice. n = 4 mice per group. d) Experimental timeline for CUMS paradigm, behavioral tests, and preparation of brain sections. e–h) Behavioral test results for control and CUMS mice. CUMS susceptible mice showed lower sucrose preference (e), a decreased central zone duration time in the OFT (f), and increased immobility time in the TST (g) and FST (h). CUMS resilient mice behaved indistinguishably from control nonstressed mice ( n = 10 mice per group). i) Fluorescence intensity of VDBP in each brain area of susceptible and resilient mice after the CUMS paradigm ( n = 4 mice per group). j) Representative immunofluorescence images of VDBP protein expression in emotion‐related brain regions in control, CUMS‐susceptible, and CUMS‐resilient mice. Scale bar = 100 µm (left) and 20 µm (right). k) Fluorescence intensity of VDBP in emotion‐related brain regions in control, CUMS‐susceptible, and CUMS‐resilient mice ( n = 4 mice per group). l,m) Representative confocal images and quantification of VDBP RNAscope signal colocalized with cell‐specific markers for neurons (NeuN), astrocytes (GFAP), and microglia (Iba1) in the PrL of control, CUMS‐susceptible, and CUMS‐resilient mice. n = 4 mice. Scale bar = µm 20 (left) and 10 µm (right). n,o) Representative confocal images (n) and quantification (o) of VDBP protein colocalized with cell‐specific markers for neurons (NeuN), astrocytes (GFAP), and microglia (Iba1) in the PrL of control, CUMS‐susceptible, and CUMS‐resilient mice. n = 4 mice. Scale bar = 20 µm (left) and 10 µm (right). Data represent the mean ± SEM. For comparisons among groups, one‐way analysis of variance (ANOVA) followed by Bonferroni post hoc tests was used. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: After being permeabilized with 0.2% Triton X‐100 and blocking with 0.5% bovine serum albumin (BSA), fixed brain tissues or cultured cells were incubated overnight at 4 °C with the following primary antibody: mouse anti‐VDBP (sc‐365441, Santa Cruz); rabbit anti‐ionized calcium binding adaptor molecule 1 (Iba‐1) (019‐19741, WAKO); rabbit anti‐neuronal nuclei (NeuN) (26975‐1‐AP, Proteintech); rabbit anti‐ glial fibrillary acidic protein (GFAP) (16825‐1‐AP, Proteintech); rabbit anti‐MAP2 (ab32454, Abcam); mouse anti‐megalin/LRP2 (sc‐515772, Santa Cruz); goat anti‐glutamate decarboxylase 67 (GAD67) (AF2086, R&D Systems); or goat anti‐CaM kinase II alpha Antibody (NBP1‐51945, Novus).

Techniques: Expressing, Immunofluorescence, Fluorescence, Control, RNAscope

Construction and validation of MG‐derived VDBP conditional knockout mice. a) Schematic of construction of VDBP fl/fl mice using the CRISPR‐Cas9 technique. The strategy for KO‐VDBP and subsequent behavioral studies is also shown. b) Southern blot of DNA from tails of F1 mice. DNA was digested using the restriction enzymes NcoI and AseI. The 3′ probe was used to detect recombination. The left homology arm (LR) probe was used to rule out random incorporation. WT: wildtype control. VDBP fl/+ F1 mice: 001, 007, 009, and 012. c) PCR validation of Cx3cr1‐Cre ER/+ ; VDBP fl/fl mice by PCR primers for 5′loxp, 3′loxp, and Cre. A1, A3, A4, A6, A7, A9, A10, A12, and A13 are the mice with the genotype Cx3cr1‐Cre ER/+ ; VDBP fl/fl . d) Immunofluorescence of VDBP in different cell types of the PrL in TAM‐treated VDBP fl/fl and KO‐VDBP mice. Blue: DAPI. Green: VDBP. Red: cell type marker (neuron: NeuN, microglia: Iba1, astrocyte: GFAP). Scale bar = 20 µm. n = 3 mice. e) Fluorescence‐activated cell sorting of microglia, neurons, astrocytes, and endothelial cells from mouse brains. f) Reverse transcriptase (RT)‐quantitative polymerase chain reaction (qPCR) of VDBP expression in different brain cell types after fluorescence‐activated cell sorting. Data were analyzed by the two‐tailed unpaired t ‐test and expressed as the mean ± SEM, n = 3 mice. Student's t ‐test was used for statistical comparisons between two groups. * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: Advanced Science

Article Title: Microglia‐Derived Vitamin D Binding Protein Mediates Synaptic Damage and Induces Depression by Binding to the Neuronal Receptor Megalin

doi: 10.1002/advs.202410273

Figure Lengend Snippet: Construction and validation of MG‐derived VDBP conditional knockout mice. a) Schematic of construction of VDBP fl/fl mice using the CRISPR‐Cas9 technique. The strategy for KO‐VDBP and subsequent behavioral studies is also shown. b) Southern blot of DNA from tails of F1 mice. DNA was digested using the restriction enzymes NcoI and AseI. The 3′ probe was used to detect recombination. The left homology arm (LR) probe was used to rule out random incorporation. WT: wildtype control. VDBP fl/+ F1 mice: 001, 007, 009, and 012. c) PCR validation of Cx3cr1‐Cre ER/+ ; VDBP fl/fl mice by PCR primers for 5′loxp, 3′loxp, and Cre. A1, A3, A4, A6, A7, A9, A10, A12, and A13 are the mice with the genotype Cx3cr1‐Cre ER/+ ; VDBP fl/fl . d) Immunofluorescence of VDBP in different cell types of the PrL in TAM‐treated VDBP fl/fl and KO‐VDBP mice. Blue: DAPI. Green: VDBP. Red: cell type marker (neuron: NeuN, microglia: Iba1, astrocyte: GFAP). Scale bar = 20 µm. n = 3 mice. e) Fluorescence‐activated cell sorting of microglia, neurons, astrocytes, and endothelial cells from mouse brains. f) Reverse transcriptase (RT)‐quantitative polymerase chain reaction (qPCR) of VDBP expression in different brain cell types after fluorescence‐activated cell sorting. Data were analyzed by the two‐tailed unpaired t ‐test and expressed as the mean ± SEM, n = 3 mice. Student's t ‐test was used for statistical comparisons between two groups. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: After being permeabilized with 0.2% Triton X‐100 and blocking with 0.5% bovine serum albumin (BSA), fixed brain tissues or cultured cells were incubated overnight at 4 °C with the following primary antibody: mouse anti‐VDBP (sc‐365441, Santa Cruz); rabbit anti‐ionized calcium binding adaptor molecule 1 (Iba‐1) (019‐19741, WAKO); rabbit anti‐neuronal nuclei (NeuN) (26975‐1‐AP, Proteintech); rabbit anti‐ glial fibrillary acidic protein (GFAP) (16825‐1‐AP, Proteintech); rabbit anti‐MAP2 (ab32454, Abcam); mouse anti‐megalin/LRP2 (sc‐515772, Santa Cruz); goat anti‐glutamate decarboxylase 67 (GAD67) (AF2086, R&D Systems); or goat anti‐CaM kinase II alpha Antibody (NBP1‐51945, Novus).

Techniques: Biomarker Discovery, Derivative Assay, Knock-Out, CRISPR, Southern Blot, Control, Immunofluorescence, Marker, Fluorescence, FACS, Reverse Transcription, Real-time Polymerase Chain Reaction, Expressing, Two Tailed Test